Christiane Kruse Fæste, Dr. rer. nat.

Toxinology Research Group
Norwegian Veterinary Institute
Ullevålsveien 68
0454 Oslo/Norway

Tel.: +47/477 53 488
E-mail: christiane.faste@vetinst.no

Research interests

Characterisation of biomarkers for toxin presence and activity by proteomic and biochemical analysis. Xenobiotics metabolism and toxicokinetics including determination of biotransformation pathways, inhibition and interaction studies, and prediction of human exposure. Risk assessment regarding exposure of humans and domestic animals from contaminated food and novel food proteins. Identification of food allergens and elucidation of allergenic potentials using immunochemical and spectrometric methods. Contact for the Norwegian National Register and Reporting System of Severe Allergic Reactions to Food. Member of the Norwegian Committee for Food Safety (Feed Panel), CEN (TC275 WG12 Food Allergens), AOAC (Food Allergen Community), and ILSI (Expert group verifying methods for food allergens reference doses).

Background

Studies in chemistry and biochemistry at the Technical University Hanover/Germany (TUH) and the Medical School Hanover/Germany. Master in pharmacology and biochemistry. Doctoral studies at the Max-Planck-Institute for Plant Breeding in Cologne/Germany and degree from the TUH in plant molecular biology. Laboratory leader in preclinical pharmacokinetics at Bayer Healthcare AG in Wuppertal/Germany. Senior scientist at the Norwegian Veterinary Institute since 2002.

Ongoing projects

SafeFish – Implications of green Fish feed for consumer safety – carry-over of plant peptides, natural toxins and bioactive compounds (NFR Havbruk2)
DONNER – Deoxynivalenol (DON) – Nervous effects reconnaissance
CiguaPIRE – Advancing global strategies and understanding on the origin of ciguatera fish poisoning in tropical oceans
iNOBox – A Technology- and Market-driven Innovation e-Toolbox towards a Sustainable, Competitive and Science-based Agri-Food Industry in Norway
NewMHCI – Changing our view on major histocompatibility complex (MHC) class I in vertebrates
FunTox – Fungi and mycotoxins in a «One Health» perspective
BioDirect – Biomarkers and Bioassays for Animal and Fish Research and Diagnostics
MycoSafeSouth – European–African partnership for safe and efficient use of mycotoxin-mitigation strategies in sub-Saharan Africa
Gyrokloramin – Chloramine treatment of watercourses for the control of Gyrodactylus salaris infestation
ISAbreed – ISA resistance in Atlantic salmon: Defining new phenotypes for accurate breeding
PromoFish – Proteomics and metabolomics as tools in aquaculture research: epidermal mucus and the Gyrodactylus salaris mystery

Recent projects

MycoPig – Mycotoxins and toxigenic fungi in Norwegian pig farming: consequences for animal health and possible intervention strategies
MycoFish
Food Allergen Methods – Development of a coordinated research program for food allergens – identification, modification and in vivo responses
LCMSMS for Food Allergens – The development of a project proposal on LCMSMS for the determination of low levels of food allergens
Anisakis simplex – Development of tools for product control and human risk evaluation in fishery value chains (Anisakis simplex)

Articles

  • Faeste CK, Pierre F, Ivanova L, Sayyari A, Massotte D. Behavioural and metabolomic changes from chronic dietary exposure to low-level deoxynivalenol reveal impact on mouse well-being. Arch Toxicol 2019, 93, 2087.
  • Oliveira CAF, Ivanova L, Solhaug A, Fæste CK. Effects of enniatin B1 on lysosomal stability and chaperone-mediated autophagy-related components in mouse embryonic fibroblasts. Mycotox Res 2019.
  • Houben G, Blom M, Alvito P, Assuncao R, Crevel R, Faeste CK, Le TM, Madsen CB, Remington B, Stroheker T, Vassilopoulou E, Verhoeckx K, Ziarovska J, Constable A. Defining the targets for the assessment of IgE-mediated allergenicity of new or modified food proteins. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2019, 127, 61.
  • Ivanova L, Denisov IG, Grinkova YV, Sligar SG, Faeste CK. Biotransformation of the mycotoxin enniatin B1 by CYP P450 3A4 and potential for drug-drug interactions. Metabolites 2019, 9.
  • Johny A, Faeste CK, Bogevik AS, Berge GM, Fernandes JMO, Ivanova L. Development and validation of a liquid chromatography high-resolution mass spectrometry method for the simultaneous determination of mycotoxins and phytoestrogens in plant-based fish feed and exposed fish. Toxins 2019, 11.
  • Sayyari A, Uhlig S, Fæste CK, Framstad T, Sivertsen T. Transfer of deoxynivalenol (DON) in placenta and milk of gestating and lactating sows. Toxins 2018, 10, 517-538.
  • Fæste CK, Ivanova L, Sayyari A, Hansen U, Sivertsen T, Uhlig S. Prediction of deoxynivalenol toxicokinetics in humans by in vitro-to-in vivo extrapolation and allometric scaling of in vivo animal data. Arch Toxicol 92, 2018, 2195-2216.
  • Sayyari A, Fæste CK, Hansen U, Uhlig S, Framstad T, Schatzmayr D, Sivertsen T. Effects and biotransformation of the mycotoxin 4-deoxynivalenol (DON) in growing pigs fed naturally-contaminated grain pelleted with and without the addition of Coriobacteriaceum DSM 11798. J. Food Add Contam A, 2018, 1-16.
  • Ivanova L, Fæste CK, Solhaug A. Role of P-glycoprotein in DON-mediated in vitro toxicity. Toxicol Lett, 2018, 284: 21-28.
  • Remington B, Blom WM, Broekman HCH, Capt A, Crevell R, Dimitrov I, Fæste CK, Fernandez-Canton R, Fernandez-Dumont A, Giavi S, Houben GF, Glenn KC, Madsen CB, Kruizinga AK, Constable A. Approaches to assess IgE mediated allergy risks (sensitization and cross-reactivity) from new or modified dietary proteins. Food Chem Tox, 2018, 112:97-107
  • Bernhoft A, Høgåsen HR, Rodenlund G, Ivanova L, Berntssen MHG, Alexander J, Eriksen GE, Fæste CK. Tissue distribution and elimination of deoxynivalenol and ochratoxin A in dietary exposed Atlantic salmon (Salmo salar). Food Add Contam A, 2017, 34:1211-1224.
  • Ivanova L, Uhlig S, Devreese M, Croubels S, Fæste CK. Biotransformation of the mycotoxin enniatin B1 in pigs: a comparative in vitro and in vivo approach. Food Chem Toxicol, 2017, 105:506-517.
  • Sundheim L, Lillegaard IT, Fæste CK, Brantsæter AL, Brodal G, Eriksen GS. Deoxynivalenol exposure in Norway, risk assessments for different human age groups. Toxins, 2017, 9:46.
  • Fæste,C.K.; Moen,A.; Schniedewind,B.; Anonsen,J.H.; Klawitter,J.; Christians,U. Development of a liquid chromatography-tandem mass spectrometry method for the quantitation of Anisakis simplex proteins in food products. J Chromatogr A, 2016, 1432, 58-72.
  • Fæste,C.K.; Plassen,C.; Løvberg,K.E.; Moen,A.; Egaas,E. Detection of proteins from the fish parasite Anisakis simplex in Norwegian farmed salmon and processed fish products. Food Anal Meth 2015, 8, 1390-1402.
  • Fæste,C.K.; Levsen,A.; Lin,A.H.; Larsen,N.; Plassen,C.; Moen,A.; Van Do,T.; Egaas,E. Fish feed as source of potentially allergenic peptides from the fish parasite Anisakis simplex (sl). Anim Feed Sci Technol 2015, 202, 52-61.
  • Ivanova,L.; Fæste,C.K.; Delezie,E.; Van Pamel,E.; Daeseleire,E.; Callebaut,A.; Uhlig,S. Presence of enniatin B and its hepatic metabolites in plasma and liver samples from broilers and eggs from laying hens. World Mycotox J 2014, 7, 167-175.
  • Bertelsen,R.J.; Fæste,C.K.; Granum,B.; Egaas,E., London, SJ, Carlsen,K.-H., Lødrup Carlsen, K.C.; Løvik,M. Food allergens in mattress dust in Norwegian homes; a potentially important source of allergen exposure. Clin Exp Allergy 2014, 44, 142-149.
  • Fæste,C.K.; Jonscher,K.R.; Dooper,M.M.W.B.; Egge-Jacobsen,W.M.; Moen,A.; Daschner,A.; Egaas,E.; Christians,U. Characterization of potential novel allergens in the fish parasite Anisakis simplex. EuPa Open Proteomics J. 2014, 4, 140-155.
  • Fæste,C.K.; Plassen,C.; Løvberg,K.E.; Moen,A.; Egaas,E. Determination of the fish parasite Anisakis simplex in Norwegian farmed salmon and processed fish products. Food Analyt. Meth. 2014, DOI 10.1007/s12161-014-0003-8.
  • Myrset, H; Fæste, C. K.; Kristiansen, P.; Dooper, M. M. W. B. Mapping of the immunodominant regions of shrimp tropomyosin Pan b 1 by human IgE binding and IgE-receptor crosslinking studies. Int. Arch. Allergy Immunol. 2013, 162, 25-38.
  • Eriksen, G. S.; Moldes-Anaya, A.; Fæste, C. K. Penitrem A and analogues: Toxicokinetics, toxicodynamics including mechanism of action and clinical significance. World Mycotoxin J. 2013, 6, 263-272.
  • Levsen, A.; Faeste, C. K.; Lin, A.H.; Van Do, T.; Florvag, E.;  Egaas, E. Consumer health risk posed by Anisakis simplex in Northern Europe – an update. Trop. Med. Int. Health 2013, 18, 49.
  • Bertelsen, R.J.; Fæste, C. K.; Lødrup Carlsen, K. C.; Egaas, E.; Carlsen, K.-H.; Granum, B.; Løvik, M. Food allergens in mattress dust in Norwegian homes; a potential important source of allergen exposure. Clin. Exp. Allergy, 2013, 3, S3, O3.
  • Ivanova, L.; Fæste, C. K.; Delezie, E.; Van Pamel, E.; Daeseleire, E.; Callebaut, A.; Uhlig, S. Presence of enniatin B and its hepatic metabolites in plasma and liver samples from broilers and eggs from laying hens. World Mycotoxin J., in press.
  • Uhlig, S.; Ivanova, L.; Fæste, C. K. Enzyme-assisted synthesis and structural characterization of the 3‑, 8‑, and 15-glucuronides of deoxynivalenol. J. Agric. Food Chem. 2013, 61, 2006-2012.
  • Namork, E.; Fæste, C. K.; Stensby, B. A., Egaas, E., Løvik, M. Severe allergic reactions to food in Norway: A ten year survey of cases reported to the Food Allergy Register. Int. J. Environ. Res. Public Health. 2011, 8, 3144-3155. Abstract
  • Fæste, C.K.; Ivanova, L.; Uhlig, S. In vitro metabolism of the mycotoxin enniatin B in different species and CYP P450 reaction phenotyping. Drug Metab. Dispos. 2011, 39, 1768-1776.
  • Ivanova, L.; Fæste, C. K.; Uhlig, S. In vitro metabolism of the depsipeptide enniatin B. Anal. Bioanal. Chem. 2011, 400, 2889-2901.
  • Faeste, C. K.; Rønning, H. T.; Christians, U.; Granum, P. E. Liquid chromatography and mass spectrometry in food allergen detection. J. Food Prot. 2011, 74 (2), 316-345.
  • Faeste, C. K.; Christians, U.; Egaas, E.; Jonscher, K. R. Characterization of potential allergens in fenugreek (Trigonella foenum-graecum) using patient sera and MS-based proteomic analysis. J. Proteomics  2010, 73 (7), 1321-1333.
  • Faeste, C. K.; Jonscher, K. R.; Sit, L.; Klawitter, J.; Lovberg, K. E.; Moen, L. H. Differentiating cross-reacting allergens in the immunological analysis of celery (Apium graveolens) by mass spectrometry. J. AOAC Int. 2010, 93 (2), 451-461.
  • Faeste, C. K.; Namork, E. Differentiated patterns of legume sensitisation in peanut-allergic patients. Food Analyt. Meth. 2010, 3, 357-362.
  • Werner, M. T.; Faeste, C. K.; Levsen, A.; Egaas, E. A quantitative sandwich ELISA for the detection of Anisakis simplex protein in seafood. Eur. Food Res. Technol. 2010, 232, 157-166.
  • Dooper, M. M.; Plassen, C.; Holden, L.; Lindvik, H.; Faeste, C. K. Immunoglobulin E cross-reactivity between lupine conglutins and peanut allergens in serum of lupine-allergic individuals. J. Investig. Allergol. Clin. Immunol. 2009, 19 (4), 283-291.
  • Faeste, C. K.; Namork, E.; Lindvik, H. Allergenicity and antigenicity of fenugreek (Trigonella foenum-graecum) proteins in foods. J. Allergy Clin. Immunol. 2009, 123 (1), 187-194.
  • Moldes-Anaya, A.; Wilkins, A. L.; Rundberget, T.; Faeste, C. K. In vitro and in vivo hepatic metabolism of the fungal neurotoxin penitrem A. Drug Chem. Toxicol. 2009, 32 (1), 26-37.
  • Faeste, C. K.; Plassen, C. Quantitative sandwich ELISA for the determination of fish in foods. J. Immunol. Methods 2008, 329 (1-2), 45-55.
  • Holden, L.; Sletten, G. B.; Lindvik, H.; Faeste, C. K.; Dooper, M. M. Characterization of IgE binding to lupin, peanut and almond with sera from lupin-allergic patients. Int. Arch. Allergy Immunol. 2008, 146 (4), 267-276.
  • Sletten, G. B. G.; Holden, L.; Egaas, E.; Faeste, C. K. Differential influence of the degree of processing on immunogenicity following proteolysis of casein and beta-lactaglobulin. Food Agri Immunol 2008, 19, 213-228.
  • Dooper, M. M.; Holden, L.; Faeste, C. K.; Thompson, K. M.; Egaas, E. Monoclonal antibodies against the candidate lupin allergens alpha-conglutin and beta-conglutin. Int. Arch. Allergy Immunol. 2007, 143 (1), 49-58.
  • Faeste, C. K.; Lovberg, K. E.; Lindvik, H.; Egaas, E. Extractability, stability, and allergenicity of egg white proteins in differently heat-processed foods. J. AOAC Int. 2007, 90 (2), 427-436.
  • Werner, M. T.; Faeste, C. K.; Egaas, E. Quantitative sandwich ELISA for the determination of tropomyosin from crustaceans in foods. J. Agric. Food Chem. 2007, 55 (20), 8025-8032.
  • Faeste, C. K.; Holden, L.; Plassen, C.; Almli, B. Sensitive time-resolved fluoroimmunoassay for the detection of hazelnut (Corylus avellana) protein traces in food matrices. J. Immunol. Methods 2006, 314 (1-2), 114-122.
  • Holden, L.; Faeste, C. K.; Egaas, E. Quantitative sandwich ELISA for the determination of lupine (Lupinus spp.) in foods. J. Agric. Food Chem. 2005, 53 (15), 5866-5871.
  • Faeste, C. K.; Lovik, M.; Wiker, H. G.; Egaas, E. A case of peanut cross-allergy to lupine flour in a hot dog bread. Int. Arch. Allergy Immunol. 2004, 135 (1), 36-39.
  • Løvik, M.; Namork, E.; Faeste, C. K.; Egaas, E. The Norwegian National Reporting System and Register of Severe Allergic Reactions to Food. Norsk Epidemiol 2004, 14, 155-160.
  • Faeste, C. K.; Wiker, H. G.; Lovik, M.; Egaas, E. Hidden shellfish allergen in a fish cake. Allergy 2003, 58 (11), 1204-1205.
  • Christians, U.; Braun, F.; Schmidt, M.; Kosian, N.; Schiebel, H. M.; Ernst, L.; Winkler, M.; Kruse, C.; Linck, A.; Sewing, K. F. Specific and sensitive measurement of FK506 and its metabolites in blood and urine of liver-graft recipients. Clin. Chem. 1992, 38 (10), 2025-2032.
  • Christians, U.; Sattler, M.; Schiebel, H. M.; Kruse, C.; Radeke, H. H.; Linck, A.; Sewing, K. F. Isolation of two immunosuppressive metabolites after in vitro metabolism of rapamycin. Drug Metab Dispos. 1992, 20 (2), 186-191.
  • Christians, U.; Braun, F.; Kosian, N.; Schmidt, M.; Schiebel, H. M.; Ernst, L.; Kruse, C.; Winkler, M.; Holze, I.; Linck, A.; . High performance liquid chromatography/mass spectrometry of FK 506 and its metabolites in blood, bile, and urine of liver grafted patients. Transplant. Proc. 1991, 23 (6), 2741-2744.
  • Christians, U.; Kruse, C.; Kownatzki, R.; Schiebel, H. M.; Schwinzer, R.; Sattler, M.; Schottmann, R.; Linck, A.; Almeida, V. M.; Braun, F.; . Measurement of FK 506 by HPLC and isolation and characterization of its metabolites. Transplant. Proc. 1991, 23 (1 Pt 2), 940-941.

Book chapters

  • Fæste CK: Allergens in Peanut, Soybean, and Lupin, Chapter 3, Food Allergens: Analysis Instrumentation and Methods, edt. Nollet L. Van Hengel A. Taylor & Francis Group, LLC, Boca Raton, Fl. USA, 2011.
  • Fæste CK: Fish allergen detection, Chapter 21, Molecular Biological and Immunological Techniques and Applications for Food Chemists, edt. Popping B, Diaz-Amigo C, Hoenicke K.  John Wiley & Sons, Ltd, 2009.
  • Fæste CK: Lupin allergen detection, Chapter 22, Molecular Biological and Immunological Techniques and Applications for Food Chemists, edt. Popping B, Diaz-Amigo C, Hoenicke K.  John Wiley & Sons, Ltd, 2009.